A validated stability indicating RP-HPLC method development and validation of for simultaneous estimation of indacaterol and glycopyrrolate in pharmaceutical dosage form

A simple, Accurate, precise method was developed for the simultaneous estimation of Indacaterol and Glycopyrronium in bulk pharmaceutical dosage form. The chromatogram run through Std Denali C18150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1%OPA: Acetonitrile taken ratio 55:45 pumped column at a flow rate 0.8 ml/min. buffer used this 0.1% OPA buffer. temperature maintained 30°C. optimized wavelength selected 230.0 nm. retention time were found to 2.323min 3.140 %RSD be 0.2% respectively. %Recovery obtained as 99.39% 99.41% LOD, LOQ values from regression equations 1.08, 3.28, 0.25, 1.47 Regression equation is y = 24501x + 7142.3, 26335x 7822.7of Glycopyrronium.Retention times decreased decreased, so simple economical that can adopted regular Quality control tests Industries.